SS-31 Peptide Dosage Guide Purity Specifications Manufacturing Sourcing for Lab Formulation

Core Molecular Specs & Technical Index

SS-31 peptide, also known as Elamipretide or MTP-131, is a mitochondrial-targeted tetrapeptide with the sequence D-Arg-Dmt-Lys-Phe-NH2. It is designed to selectively bind to cardiolipin in the inner mitochondrial membrane, stabilizing the electron transport chain and reducing reactive oxygen species production. For B2B buyers in cosmetic formulation and laboratory research, understanding the precise technical specifications is critical for reproducible results and regulatory compliance.

• Molecular Formula & Weight: C38H60N10O8, exact mass 784.46 g/mol. The peptide is supplied as a lyophilized powder with a net peptide content of ≥95%.
• Purity Grade: HPLC purity ≥98% (standard research grade) and ≥99% (ultra-pure for advanced formulation). Impurity profiles are controlled to <0.5% for each single impurity.
• Solubility Profile: Soluble in water at 10 mg/mL, in PBS (pH 7.4) at 20 mg/mL, and in DMSO at 50 mg/mL. For cosmetic formulations, pre-dissolve in sterile water or buffer before incorporation.
• Storage Conditions: Lyophilized powder stable for 24 months at -20°C, protected from light and moisture. Reconstituted solutions must be used within 72 hours when stored at 2-8°C.
• Appearance & Counterion: White to off-white lyophilized powder, typically supplied as trifluoroacetate (TFA) salt. Residual TFA content is controlled to <5% by weight.

Industry data from the 2023 Peptide Therapeutics Symposium indicates that SS-31 peptide dosage precision within ±2% of target concentration is required for reproducible mitochondrial function assays. Labs using peptides with purity below 95% reported 40% higher variability in ATP production measurements.

Manufacturing & Quality Control

SS-31 peptide is manufactured using solid-phase peptide synthesis (SPPS) on a Rink amide resin, employing Fmoc chemistry with HBTU/HOBt activation. The synthesis cycle includes coupling, deprotection, and capping steps to minimize deletion sequences. After cleavage from the resin, the crude peptide undergoes preparative reverse-phase HPLC purification using a C18 column with a water/acetonitrile gradient containing 0.1% TFA. The final product is lyophilized under controlled conditions to maintain consistent cake structure and residual moisture below 3%.

Quality control protocols include three tiers of testing. First, analytical HPLC with UV detection at 220 nm and 280 nm confirms purity and retention time consistency. Second, mass spectrometry (ESI-MS or MALDI-TOF) verifies molecular weight within ±0.5 Da. Third, amino acid analysis after acid hydrolysis quantifies peptide content and confirms the correct stoichiometry of D-Arg, Dmt, Lys, and Phe. Each batch is accompanied by a Certificate of Analysis (COA) that includes these parameters.

• Third-Party Testing: Independent labs perform endotoxin testing (LAL method, <1 EU/mg), bioburden testing (TAMC/TYMC), and heavy metal analysis (ICP-MS, <10 ppm total metals).
• Certification Documentation: COA, MSDS, and batch-specific HPLC chromatograms are provided. For cosmetic-grade material, a non-animal origin statement and GMP compliance letter are available.
• Stability Studies: Accelerated stability at 40°C/75% RH for 4 weeks shows <2% degradation. Real-time stability at -20°C extends to 24 months with no significant loss of potency.

Commercial Application Scenarios

SS-31 peptide is utilized across three primary B2B segments: cosmetic formulation, laboratory research, and bulk wholesale distribution. In cosmetic formulation, the peptide is incorporated into serums and creams at concentrations ranging from 0.1% to 1.0% (w/w). The formulation must include antioxidants like vitamin E or ferulic acid to protect the peptide from oxidation. Typical batch sizes for cosmetic labs range from 1 kg to 10 kg per month, with the peptide pre-dissolved in a glycol or water phase before emulsification.

For laboratory research, SS-31 peptide dosage is critical for in vitro and in vivo studies. In cell culture models, working concentrations of 1–10 µM are standard, with incubation times of 24–72 hours. For animal studies, intravenous or intraperitoneal administration at 0.5–3 mg/kg body weight is common, with dosing schedules ranging from daily to every other day. Researchers must prepare fresh solutions for each experiment to avoid peptide aggregation or oxidation.

Bulk wholesale buyers typically order 100 g to 500 g per shipment for inventory or distribution. These orders require custom packaging in sealed, nitrogen-flushed vials or bags with desiccant. Documentation for customs clearance includes the COA, MSDS, and a commercial invoice with harmonized tariff code 2934.99.9000 (peptides for research purposes). Lead times for bulk orders are 2–4 weeks, depending on synthesis scale and purification requirements.

ss-31 peptide dosage VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing SS-31 peptide for bulk orders, buyers must avoid common pitfalls that compromise quality and regulatory compliance. The first pitfall is accepting peptides without a Certificate of Analysis. Reputable suppliers provide batch-specific COAs that include HPLC purity, MS confirmation, peptide content, and residual solvent levels. Without this documentation, the peptide cannot be verified for its intended use.

The second pitfall is underestimating the importance of packaging. SS-31 peptide is hygroscopic and sensitive to oxidation. Bulk orders should be packaged in amber glass vials or aluminum foil bags with nitrogen flushing and desiccant. Avoid suppliers that use plastic containers or non-sealed packaging, as moisture ingress can degrade the peptide within weeks.

Third, buyers should verify the supplier's manufacturing scale and lead time. Small-scale suppliers may not have the capacity to produce consistent quality for orders above 50 g. Request a sample batch (1–5 g) for in-house testing before committing to a large order. The sample should be tested for purity, solubility, and biological activity in your specific assay or formulation.

• Selection Checklist: Confirm purity ≥98%, request COA and MSDS, verify packaging integrity, test sample batch, check lead time (2–4 weeks), and review supplier's GMP or ISO certification.
• Documentation Requirements: Commercial invoice, packing list, COA, MSDS, and non-animal origin statement for cosmetic use.
• Shipping Conditions: Dry ice or gel packs for international shipments. Ensure the peptide remains frozen during transit.

Core Product Advantages

Our SS-31 peptide offers three distinct advantages for B2B buyers: guaranteed purity, exceptional stability, and comprehensive technical support. The purity of ≥98% by HPLC ensures that each batch contains minimal deletion sequences or oxidation byproducts, which is critical for reproducible results in both formulation and research. The peptide content is verified by amino acid analysis, so the ss-31 peptide dosage can be calculated accurately without correction for counterion or moisture.

Stability is enhanced through optimized lyophilization conditions and packaging. The peptide retains >95% potency after 24 months at -20°C and >90% after 6 months at 4°C. For reconstituted solutions, we provide validated stability data in common buffers (PBS, water, DMSO) to guide end-user handling. This reduces waste and ensures consistent performance across multiple experiments or production batches.

Technical support includes application notes for formulation development, solubility optimization, and assay design. Our team of peptide chemists and formulation scientists can assist with custom packaging, labeling, and documentation for regulatory submissions. For bulk orders, we offer volume discounts and flexible payment terms. Each order is tracked with a unique batch number for full traceability from synthesis to delivery.

Frequently Asked Questions

Q1: What is the recommended ss-31 peptide dosage for cosmetic serum formulations?
For cosmetic serums, the typical ss-31 peptide dosage is 0.1% to 1.0% (w/w) of the final formulation. Start with 0.5% for initial stability and efficacy testing. The peptide should be dissolved in the water phase at 40–50°C before emulsification. Avoid prolonged heating above 60°C to prevent degradation. Include antioxidants like 0.1% vitamin E to protect the peptide during shelf life.

Q2: How do I verify the purity of SS-31 peptide from a new supplier?
Request a 1–5 g sample and perform analytical HPLC using a C18 column with a 5–60% acetonitrile gradient in 0.1% TFA over 30 minutes. The main peak should elute at a retention time consistent with the reference standard (typically 18–22 minutes). Compare the UV spectrum at 220 nm and 280 nm. For confirmation, send the sample for ESI-MS analysis; the expected m/z is 785.5 [M+H]+. Reject any batch with purity below 95% or with multiple impurity peaks above 1%.

Q3: What documentation is required for importing SS-31 peptide for research use?
For research use, the importer must provide a commercial invoice with the product description, quantity, unit price, and harmonized tariff code 2934.99.9000. The supplier must include a Certificate of Analysis (COA) with batch number, purity, and test methods. A Material Safety Data Sheet (MSDS) is required for shipping. Some countries may require an end-user statement confirming the peptide is for research only and not for human consumption. Check with your local customs broker for specific import regulations.