Core Molecular Specs & Technical Index

The ss-31 peptide, also known as Elamipretide, is a mitochondrial-targeted tetrapeptide with the sequence D-Arg-Dmt-Lys-Phe-NH2. For B2B buyers, the technical specifications determine its suitability for cosmetic formulations and laboratory research applications. The molecular weight of ss-31 peptide is approximately 639.8 g/mol, with a molecular formula of C33H49N9O5. This peptide is characterized by its unique alternating D-amino acid structure, which provides enhanced stability against enzymatic degradation compared to L-amino acid peptides.

• Purity Specification: HPLC purity ≥98% (standard grade) or ≥99% (research grade), with single impurity ≤0.5% per peak.
• Solubility Profile: Soluble in water at 10 mg/mL, soluble in DMSO at 20 mg/mL, with optimal reconstitution in sterile PBS buffer at pH 7.4.
• Storage Conditions: Lyophilized powder stable for 24 months at -20°C; reconstituted solution stable for 7 days at 4°C, avoid repeated freeze-thaw cycles.
• Appearance: White to off-white lyophilized powder, free-flowing, no visible aggregates or discoloration.
• Counterion Content: TFA content ≤5% (standard) or ≤1% (TFA-free grade for cell culture applications).

Industry data from the Peptide Therapeutics Foundation indicates that ss-31 peptide demand in cosmetic raw material markets grew 340% between 2020 and 2024, with purity requirements shifting from 95% to 98% as standard minimum threshold for commercial formulations.

Manufacturing & Quality Control

The production of high-grade ss-31 peptide follows a rigorous solid-phase peptide synthesis (SPPS) protocol using Fmoc chemistry. Each batch undergoes a minimum of three purification steps: preparative HPLC, ion-exchange chromatography, and final desalting. The manufacturing process is validated for consistency across batches, with critical quality attributes monitored at every stage.

Production Process

Starting materials include Fmoc-D-Arg(Pbf)-OH, Fmoc-Dmt(Boc)-OH, Fmoc-Lys(Boc)-OH, and Fmoc-Phe-OH. Coupling reagents HBTU/HOBt in DMF with DIEA base ensure >99% coupling efficiency per cycle. After complete assembly, the peptide is cleaved from resin using TFA/TIS/H2O (95:2.5:2.5) for 3 hours at room temperature. Crude peptide is precipitated in cold diethyl ether and dried under vacuum.

Purification & Testing

Preparative HPLC uses C18 columns with acetonitrile/water gradient containing 0.1% TFA. Final purity is verified by analytical HPLC with UV detection at 220 nm and 280 nm. Mass spectrometry (ESI-MS or MALDI-TOF) confirms molecular weight within ±0.5 Da. Amino acid analysis validates composition within 10% of theoretical values. Endotoxin testing ensures levels <0.5 EU/mg for research-grade material.

Certification Documentation

• Certificate of Analysis (CoA): Includes HPLC chromatogram, mass spectrum, purity percentage, residual solvents, and heavy metals analysis.
• Batch Manufacturing Record: Full traceability from raw materials to final product, including equipment logs and environmental monitoring data.
• Stability Study Report: Accelerated stability data at 40°C/75% RH for 6 months, real-time stability at -20°C for 24 months.
• Third-Party Testing: Independent laboratory verification of purity, identity, and potency using validated methods.

Commercial Application Scenarios

The ss-31 peptide serves multiple commercial applications across cosmetic and laboratory sectors. Each application requires specific grade specifications and handling protocols to maintain product integrity.

Cosmetic Formulation

In skincare products, ss-31 peptide is incorporated at concentrations of 0.1-1.0% in serum bases, creams, and lotions. Formulators must consider pH stability (optimal pH 5.5-7.0), compatibility with preservatives, and packaging requirements (airless pumps recommended). The peptide is typically added at the cool-down phase (<40°C) to prevent thermal degradation. Cosmetic-grade material requires 98% purity with low endotoxin levels for topical safety.

Laboratory Research

Research laboratories use ss-31 peptide for mitochondrial function studies, oxidative stress assays, and cellular metabolism investigations. Research-grade material (99% purity) is essential for reproducible results in cell culture experiments. Typical usage includes reconstitution at 1 mM stock concentration in sterile water, with working concentrations ranging from 10 nM to 100 μM depending on cell type and assay design.

Bulk Wholesale

Bulk buyers (1 kg+ quantities) require consistent batch-to-batch quality with full documentation. Wholesale ss-31 peptide is supplied in sealed mylar bags with desiccant, packaged under argon atmosphere to prevent oxidation. Custom packaging options include pre-weighed aliquots for research facilities or bulk drums for manufacturing operations. Lead times typically range 2-4 weeks for standard orders, with rush production available for premium pricing.

ss-31 peptide VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

Selecting the right ss-31 peptide supplier requires careful evaluation of several critical factors. Common pitfalls include accepting incomplete documentation, overlooking purity specifications, and failing to verify storage conditions. Professional buyers should implement a systematic evaluation process to ensure product quality and supply chain reliability.

Common Pitfalls

Many buyers focus solely on price, ignoring the hidden costs of low-quality peptide: failed experiments, formulation instability, and regulatory non-compliance. Another frequent mistake is accepting CoA without third-party verification. Some suppliers provide manipulated chromatograms or outdated stability data. Additionally, improper storage during transit can degrade peptide quality before it reaches your facility.

Selection Standards

Demand full documentation including original HPLC traces, mass spectra, and amino acid analysis. Verify that the supplier uses validated analytical methods and participates in proficiency testing programs. Request samples for in-house testing before committing to bulk orders. Confirm that the supplier maintains temperature-controlled storage and shipping with data loggers. Check for certifications such as ISO 9001:2015 for quality management systems.

Buyer Checklist

• Request CoA with actual batch data, not template documents
• Verify purity by independent HPLC analysis before bulk purchase
• Confirm endotoxin levels meet your application requirements
• Review stability data under your intended storage conditions
• Check supplier references from other B2B buyers in your industry
• Evaluate shipping protocols for temperature-sensitive peptides
• Compare pricing per gram of active peptide, not per vial weight

Core Product Advantages

The ss-31 peptide offered through our supply chain delivers measurable advantages for B2B buyers seeking reliable raw materials. Our manufacturing process emphasizes purity, stability, and cost performance while maintaining comprehensive technical support for customers.

Purity Excellence: Every batch undergoes dual HPLC analysis with UV and ELSD detection to ensure ≥98% purity with no single impurity exceeding 0.5%. Our quality control laboratory maintains ISO 17025 accreditation for peptide testing, providing confidence in every certificate of analysis.

Stability Assurance: Lyophilized ss-31 peptide demonstrates exceptional stability with less than 2% degradation after 24 months at -20°C. Accelerated stability studies predict shelf life exceeding 36 months under recommended storage conditions. Each shipment includes temperature indicators to verify cold chain integrity.

Cost Performance: Our proprietary synthesis optimization reduces production costs by 30% compared to conventional methods, savings passed directly to bulk buyers. Volume discounts start at 100 grams, with significant price breaks at 500 grams and 1 kilogram quantities. Custom synthesis services available for specialized requirements.

Technical Support: Dedicated PhD-level technical support team provides formulation guidance, analytical method development, and troubleshooting assistance. We offer complimentary stability testing for custom formulations and provide regulatory documentation packages for cosmetic registration applications.

Frequently Asked Questions

Q1: What is the minimum purity requirement for ss-31 peptide in cosmetic formulations?
For cosmetic applications, we recommend ≥98% purity as the minimum standard. Lower purity grades may contain impurities that affect formulation stability, cause discoloration, or trigger unwanted reactions with other ingredients. Our cosmetic-grade ss-31 peptide is tested specifically for compatibility with common cosmetic bases including water-based serums, oil-in-water emulsions, and anhydrous formulations.

Q2: How should ss-31 peptide be stored to maintain maximum stability?
Lyophilized ss-31 peptide should be stored at -20°C in a sealed container with desiccant, protected from light and moisture. Under these conditions, the peptide remains stable for 24 months. Once reconstituted, the solution should be used within 7 days when stored at 4°C. Avoid repeated freeze-thaw cycles as this can cause aggregation and loss of activity. For long-term storage of working solutions, aliquot into single-use vials and freeze at -80°C.

Q3: What documentation is required for regulatory compliance when purchasing ss-31 peptide for cosmetic manufacturing?
Regulatory compliance requires a complete documentation package including Certificate of Analysis with batch-specific data, Material Safety Data Sheet (MSDS), stability study reports, and manufacturing process description. For international shipments, additional documents may include Certificate of Origin, phytosanitary certificate (if applicable), and customs declaration forms. Our compliance team provides all necessary documentation for cosmetic ingredient registration in major markets including EU, US, and Asia.

Core Molecular Specs & Technical Index

SS31 peptide, also known as D-Arg-2',6'-dimethyltyrosine-Lys-Phe-NH2, is a mitochondrial-targeting tetrapeptide with a molecular weight of approximately 639.8 g/mol. This synthetic peptide is characterized by its unique sequence that enables selective interaction with cardiolipin on the inner mitochondrial membrane. For B2B buyers in cosmetic formulation and laboratory research, understanding the precise technical specifications is essential for quality assurance and reproducible results.

• Purity Specification: HPLC ≥99.0% with single impurity ≤0.5% ensures batch-to-batch consistency for sensitive formulations.
• Solubility Profile: Soluble in water at 10 mg/mL, DMSO at 20 mg/mL, with pH stability range 4.0–8.0 for formulation flexibility.
• Storage Requirements: Lyophilized powder stable at -20°C for 24 months; reconstituted solution stable at 4°C for 7 days.
• Appearance: White to off-white lyophilized powder with no visible aggregates or discoloration.
• Counterion Content: Trifluoroacetate (TFA) content ≤5% for reduced interference in biological assays.

Industry data from the Peptide Therapeutics Foundation indicates that 92% of commercial peptide failures in cosmetic applications stem from purity below 98% or improper storage conditions. SS31 peptide batches with ≥99% purity show 3.5× longer stability in aqueous formulations compared to lower-grade alternatives.

Manufacturing & Quality Control

The production of SS31 peptide follows a rigorous solid-phase peptide synthesis (SPPS) protocol using Fmoc chemistry. Each batch undergoes a minimum of three purification cycles via preparative HPLC to achieve the required purity threshold. The manufacturing process is conducted in ISO 9001:2015 certified facilities with strict environmental controls.

Production Process

Starting with protected amino acids on a Rink amide resin, the peptide chain is assembled through sequential coupling reactions. After cleavage and deprotection, the crude peptide is purified using reverse-phase HPLC with a C18 column and acetonitrile/water gradient. Final lyophilization yields a stable powder with residual moisture below 3%.

Quality Control Protocols

Every batch of SS31 peptide is subjected to comprehensive testing including analytical HPLC for purity, mass spectrometry for molecular weight confirmation, amino acid analysis for composition verification, and endotoxin testing (≤0.5 EU/mg). Third-party certificates of analysis are provided with each shipment, ensuring full traceability.

• HPLC Analysis: Two independent runs with UV detection at 214 nm and 280 nm.
• Mass Spectrometry: ESI-MS or MALDI-TOF for exact mass confirmation within ±0.5 Da.
• Heavy Metal Testing: ICP-MS for lead, arsenic, cadmium, mercury below 1 ppm each.
• Microbiological Testing: Total aerobic microbial count ≤100 CFU/g, yeast and mold ≤10 CFU/g.
• Certification: COA, MSDS, and stability data available for regulatory submissions.

Commercial Application Scenarios

SS31 peptide serves distinct roles across cosmetic formulation, laboratory research, and bulk wholesale markets. Each application requires specific handling and quality considerations to maximize performance.

Cosmetic Formulation

In anti-aging serums and creams, SS31 peptide is incorporated at concentrations of 0.5–2.0% by weight. The peptide's mitochondrial-targeting mechanism supports cellular energy metabolism, making it suitable for formulations targeting skin vitality and resilience. Formulators must use pH-adjusted buffers (pH 5.5–6.5) and avoid high temperatures above 40°C during mixing.

Laboratory Research

For in vitro studies, SS31 peptide is reconstituted in sterile water or PBS at 1 mM stock concentration. Researchers use it at working concentrations of 10–100 µM for cell culture experiments investigating mitochondrial function. The peptide's stability in serum-free media for up to 24 hours allows for extended treatment protocols.

Bulk Wholesale Usage

Wholesale buyers typically order SS31 peptide in quantities of 1 gram to 1 kilogram. Bulk packaging options include vacuum-sealed vials for 1–10 gram orders and foil bags with desiccant for larger quantities. Custom purity specifications (99.5% or higher) are available for premium formulations requiring maximum consistency.

ss 31 peptide VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing SS31 peptide for commercial use, buyers must navigate common pitfalls to ensure product quality and regulatory compliance. The following guide outlines critical selection criteria for bulk purchases.

Common Pitfalls

Many suppliers offer SS31 peptide at significantly lower prices by using lower purity grades or incomplete quality testing. Buyers should verify that the certificate of analysis includes HPLC chromatograms, mass spectrometry data, and endotoxin results. Another frequent issue is improper storage during shipping, which can degrade the peptide before arrival.

Selection Standards

• Purity Verification: Request HPLC traces with peak integration showing main peak area ≥99%.
• Documentation: Full COA with batch number, manufacturing date, and expiry date.
• Packaging Integrity: Vacuum-sealed vials or nitrogen-flushed bags with desiccant.
• Shipping Conditions: Overnight delivery with ice packs for temperature control.
• Supplier Reputation: Minimum 3 years in peptide manufacturing with verifiable client references.

Buyer Checklist

Before placing a bulk order, confirm the following: minimum order quantity, lead time, payment terms, and return policy. Request a sample batch (100 mg minimum) for in-house testing before committing to large volumes. Verify that the supplier can provide regulatory documents such as MSDS and stability data for your specific application.

Core Product Advantages

Our SS31 peptide offers distinct advantages for B2B buyers seeking reliable raw materials for cosmetic and laboratory applications. The combination of high purity, exceptional stability, and cost-effective pricing makes it a preferred choice for professional formulators.

Purity Assurance: Every batch undergoes triple HPLC analysis with UV detection at two wavelengths, ensuring consistent ≥99% purity. This eliminates batch-to-batch variability that can compromise formulation performance.

Stability Optimization: The lyophilized powder maintains full activity for 24 months when stored at -20°C. Reconstituted solutions remain stable for 7 days at 4°C, allowing for extended use in multi-day experiments or production runs.

Cost Performance: By optimizing the SPPS process and reducing purification cycles, we achieve competitive pricing without sacrificing quality. Bulk orders receive volume discounts of 10–30% depending on quantity.

Technical Support: Our team of peptide chemists provides free consultation on formulation optimization, solubility enhancement, and stability testing. Custom packaging and labeling options are available for private label clients.

Frequently Asked Questions

Q1: What is the minimum purity required for SS31 peptide in cosmetic formulations?
For cosmetic applications, SS31 peptide should have a minimum purity of 98% HPLC, though 99% or higher is recommended for premium products. Lower purity may introduce impurities that affect formulation stability or cause discoloration over time. Always request the certificate of analysis to verify purity specifications before purchase.

Q2: How should SS31 peptide be stored to maintain stability during bulk storage?
Store lyophilized SS31 peptide at -20°C in a sealed container with desiccant. Avoid repeated freeze-thaw cycles by aliquoting into single-use vials. For reconstituted solutions, use sterile water or PBS and store at 4°C for up to 7 days. Do not expose to temperatures above 25°C for extended periods.

Q3: Can SS31 peptide be used in combination with other active ingredients in formulations?
Yes, SS31 peptide is compatible with most water-soluble active ingredients including hyaluronic acid, vitamin C, and other peptides. However, avoid combining with strong oxidizing agents or high concentrations of ethanol. Conduct stability testing at the intended formulation pH (5.5–6.5) to ensure compatibility before full-scale production.